Rules to minimise tests on animals in India circulated

With support from the People for Ethical Treatment of Animals (PETA) India, the Chemicals and Fertilisers Ministry's Department of Chemicals and Petrochemicals has circulated its updated draft of Chemicals (Management and Safety) Rules, 20xx, among stakeholders, that includes provisions to minimise testing on animals.

In India, animals are killed every year while testing the safety of chemicals vis-a-vis human health and environment. In these tests, animals may be forced to consume food or water laced with a chemical, have the chemical pumped into their stomachs, or be forced to inhale the chemical before getting killed.

As the only animal protection group to participate in the stakeholder consultation meeting on May 11 to finalise the draft Rules, PETA India made many recommendations for using reliable and relevant non-animal testing approaches that protect human health and environment, which have since been incorporated in the draft.

To avoid repeat testing, the rules require that existing scientific evidence be considered prior to conducting any new tests and data submitted for the registration of substances in foreign jurisdictions will also be accepted by the Chemical Regulatory Division.

Furthermore, the Organisation for Economic Cooperation and Development's (OECD) non-animal methods must be used to derive the required data, wherever possible, with tests on vertebrates undertaken only as a last resort, as proposed by PETA India.

The person registering a substance will also be required to propose a testing strategy for approval by the division prior to any new test.

The draft rules, which will supersede two existing sets of rules -- The Manufacture, Storage and Import of Hazardous Chemical Rules, 1989; and The Chemical Accidents (Emergency Planning, Preparedness and Response) Rules, 1996 -- describe a multifaceted programme to identify and manage risks associated with the use of imported chemicals or those manufactured in India.

"We appreciate the government's step towards modernising the regulatory framework for chemicals, and there are ample opportunities to further minimise tests on animals," said PETA India Research Associate Dr Ankita Pandey.

"The new Rules, once implemented with PETA India's recommendations, will save animals from getting poisoned and killed in unreliable tests. PETA India will continue to work with the Ministry to ensure that the legislations on chemicals are most scientifically advanced in the world and that non-animal testing approaches are used wherever possible."

As proposed by PETA India, further opportunities to minimise tests on animals include implementation of 'one-substance, one-registration' rule that would mandate data-sharing between multiple persons registering the same chemical substance to ensure no duplication of animal tests, if required for registration.

Other steps involve establishment of a non-animal methods unit in addition to many other units under the Rules to provide scientific support to the division by providing regular updates on availability of OECD's valid non-animal methods, and review of technical dossiers and testing proposals.

Source: https://southasiamonitor.org

Read More........→October 04, 2020

IISc scientists develop technique to study cancer cells

An interdisciplinary team of researchers from the Indian Institute of Science (IISc) has made a breakthrough in the field of identifying cancer cells by using a 3D tumour model and magnetically-driven nanomotors to probe the microenvironment of these cells. The team consists of researchers from the Centre for Nano Science and Engineering (CeNSE) and Department of Molecular Reproduction, Development and Genetics (MRDG).

"What came as a beautiful surprise was that within such a milieu, we found that aggressive cancer cells ended up remodelling their surroundings by making them stickier, and richer in specific charged sugars," Assistant Professor at MRDG and one of the senior authors Ramray Bhat observed.

"This charging can potentially be used to target and kill tiny populations of cancer cells hidden among their normal counterparts, for which we are extending these studies to living animals," he added.

In their work, published in Angewandte Chemie, the team has steered helical nanomotors remotely via an external magnetic field through the tumour model to sense, map and quantify changes in the cellular environment.

"We believe these findings will find use as targeting strategies in future in-vivo applications, in quantifications of cancer aggressions and as biophysical probes to study the extracellular environment of cancer," the team concluded in the journal.

"The model comprises both healthy and cancer cells embedded within a reconstituted basement membrane matrix, and mimics the breast cancer environment," the paper published Angewandete Chemie stated.

While a note released by the IISc claimed that this study also highlights a new way of targeting cancer cells by maneuvering nanomotors inside a tumour and waiting for them to localise in the vicinity of the cancerous site.

"We tried driving the nanomotors toward cancer cells in a tumour model and observed them getting stuck to the matrix near cancer cells, but this was not observed near normal cells," the co-first author and PhD student at the CeNSE Debayan Dasgupta said.

The IISc note further claimed that the extracellular matrix (ECM) is a complex 3D network of proteins and carbohydrates secreted by living cells into their neighbourhood.

"However, when cancer cells secrete fresh material into the ECM, it disrupts the chemical and physical composition of the native ECM surrounding healthy cells, degrading the local environment.

Therefore, understanding how the cellular microenvironment is altered due to cancer cells and measuring these changes quantitatively could be vital in understanding the progression of cancer," Dasgupta disclosed.

In the current study, the researchers discovered that as the nanomotors approached the cancer cell membrane, they stuck to the matrix more strongly than they would to normal cells. To measure how strongly the nanomotors bound to the matrix, the team calculated the magnetic field strength required to overcome the adhesive force, and move forward.

"This means that the cancer cells are doing something. So, we did some measurements and discovered that the adhesive force depended on the type of cells, the strength of interaction and also which side of the cell the nanomotor approached," Associate Professor at CeNSE and one of the senior authors Ambarish Ghosh explained.

"In the end, we really ended up discovering a physical property of an important biological environment," he said.

The reason why the nanomotors appear to stick to the cancer cells better is their charged ECM. This may be due to the presence of 2, 3-linked sialic acid, a sugar-conjugated molecule which confers a negative charge on the cancer cell environment, the researchers found. They visualised the distribution of these sugars using fluorescent markers and found that sialic acids were distributed up to 40 micrometres from the cancer cell surface -- the same distance until which the nanomotors experienced strong adhesion.

To counter this adhesive effect, the team coated the nanomotors with Perfluorooctyltriethoxysilane (PFO) which shielded them from the charged environment.

"The coated nanomotors did not stick to the matrix near the cancer cells, whereas the uncoated motors clung to the matrix, confirming the fact that the negatively charged cancer microenvironment interacts with the incoming nanomotors, rendering them immobile," the paper published Angewandete Chemie read.

Prof. Ghosh further added that they have demonstrated how investigation of maneuverability of the nanomotors can be used to spatially differentiate between cancerous and non-cancerous cell niches that were engineered to coexist within tissue-like environment.

(IANS) Source: https://southasiamonitor.org/

Read More........→October 03, 2020