In World First, Scientists Share What Was Almost Certainly a Conversation with a Humpback Whale

Photo by Christopher Michel, CC license

In a world first, marine biologists were able to have a discourse with a humpback whale, pushing out the boundaries of cooperation and understanding that could be possible between our two species.

An adult female humpback whale, known as Twain, in Southeast Alaska, was located along with a group of whales and called with a recording of another humpback’s “whup/throp” call.

The recording was made by whales of the same group the day before, but the team didn’t know if the calls recorded were made by the same whale or were part of an exchange between two or more whales. To find out, the researchers conducted the trial on two days, with the first to find out if the whup calls they had recorded would be socially acceptable.

It turned out that whatever the whale(s) had been saying the day before was appropriate as far as Twain was concerned, and after the team broadcast the playback, she drifted away from her group and participated both physically and acoustically in three phases of interaction with the crew and their boat including periods of engagement, agitation, and disengagement.

First she called back, then she circled the boat three times, surfaced, and dived again. After this interaction, she gradually left.

Twain’s whup calls on day 2 and the whup calls recorded on day 1 were acoustically analyzed for both spectral and temporal features, specifically the inter-call interval, or latency between calls as measured by the time difference between the preceding call’s offset and the subsequent call’s onset.

The scientists determined that this metric would be able to determine both arousal and valence, thereby allowing them to get some idea of the emotional content of the exchange. The results indicated that substantial variation was found among the latencies in Twain’s calling behavior, which they took to mean excitement or arousal.

“After playing the contact call three times, we got this huge response,” said Brenda McCowan told the BBC. “Then, to keep the animal engaged, I started trying to match the latency of her calls to our calls. So, if she waited 10 seconds, I waited 10 seconds. We ended up matching each other. We did this 36 times over a 20-minute period.”

Twain’s calls were significantly shorter during engagement than either during the period defined as agitation, when she was circling the boat ejecting air through her blowhole, or as she was leaving.

The BBC reports that the songs of the humpback whale are thought to be among the most complex in the animal kingdom.A strong point of the whole experiment was that the results were determined with a degree of blinding—via independent, uninformed observers reporting on surface behavior and respiratory activity of the interacting whale.In World First, Scientists Share What Was Almost Certainly a Conversation with a Humpback Whale

Read More........→April 16, 2024

Scientists Discover Potential HIV Cure that Eliminates Disease from Cells Using CRISPR-Cas Gene Editing

HIV-1 virus particles under electron micrograph with H9 T-cells (in blue) – Credit: National Institute of Allergy and Infectious Diseases

A new study has unveiled a likely future cure for HIV which uses molecular scissors to ‘cut out’ HIV DNA from infected cells. To cut out this virus, the team used CRISPR-Cas gene editing technology—a groundbreaking method that allows for precise alterations to a patient’s genome, for which its inventors won the Nobel Prize in Chemistry in 2020. One of the significant challenges in HIV treatment is the virus’s ability to integrate its genome into the host’s DNA, making it extremely difficult to eliminate—but the CRISPR-Cas tool provides a new means to isolate and target HIV DNA. Because HIV can infect different types of cells and tissues in the body, each with its own unique environment and characteristics, the researchers are searching for a way to target HIV in all of these situations. In this study, which is to be presented ahead of this year’s European Congress of Clinical Microbiology and Infectious Diseases, the authors used CRISPR-Cas and two guide RNAs against “conserved” HIV sequences. They focused on parts of the virus genome that stay the same across all known HIV strains and infected T cells. Their experiments showed outstanding antiviral performance, managing to completely inactivate HIV with a single guide RNA and cut out the viral DNA with two guide RNAs. “We have developed an efficient combinatorial CRISPR-attack on the HIV virus in various cells and the locations where it can be hidden in reservoirs, and demonstrated that therapeutics can be specifically delivered to the cells of interest,” said Associate professor Elena Herrera Carrillo from the University of Amsterdam AMC. “These findings

HIV AIDS virus (in yellow) infecting a human cell – Credit: National Cancer Institute

represent a pivotal advancement towards designing a cure strategy.” The team has a long way to go before their cure will be available to patients, but said, “These preliminary findings are very encouraging’. Currently, HIV can be kept in check with anti-retroviral medication, but no one has actually been cured—although three patients receiving stem cell transplants for blood cancer were subsequently declared free of the disease when their HIV became undetectable. “We hope to achieve the right balance between efficacy and safety of this CURE strategy,” said Dr. Carrillo. “Only then can we consider clinical trials of ‘cure’ in humans to disable the HIV reservoir.“Our aim is to develop a robust and safe combinatorial CRISPR-Cas regimen, striving for an inclusive ‘HIV cure for all’ that can inactivate diverse HIV strains across various cellular contexts. Scientists Discover Potential HIV Cure that Eliminates Disease from Cells Using CRISPR-Cas Gene Editing

Read More........→March 26, 2024