Daily Protein Science

An international group of scientists studied the effects of 17 different lifespan-extending interventions on gene activity in mice and discovered genetic biomarkers of longevity. The results of their study were published in the journal Cell Metabolism.
Nowadays, dozens of interventions are known that extend the lifespan of various living organisms ranging from yeast to mammals. They include chemical compounds (e.g. rapamycin), genetic interventions (e.g. mutations associated with disruption of growth hormone synthesis), and diets (e.g. caloric restriction). Some targets of these interventions have been discovered. However, there is still no clear understanding of the systemic molecular mechanisms leading to lifespan extension.
A group of scientists from Skoltech, Moscow State University and Harvard University decided to fill this gap and identify crucial molecular processes associated with longevity.
To do so, they looked at the effects of various lifespan-extending interventions on the activity of genes in a mouse, a commonly used model organism closely related to humans.
The scientists identified a group of genes, whose activity was associated with longevity in response to various interventions, serving as biomarkers of lifespan extension.
"In our lab, we subjected mice of different sexes and ages to 8 longevity interventions and analyzed gene expression changes induced by these treatments. After aggregating our data with the datasets published by other groups, we obtained gene activity profiles of 17 interventions. Although in general the effects produced by individual treatments turned out to be rather specific, a certain group of genes changed its expression in a similar way in response to different lifespan-extending interventions," says the first author of the study, Alexander Tyshkovskiy.
The scientists then applied the discovered biomarkers to search for other interventions with the same effect on their activity and, therefore, high potential for lifespan extension. In their work, the researchers identified several such treatments, including chronic hypoxia and chemical compounds, such as antioxidant ascorbyl-palmitate and mTOR inhibitor, KU-0063794.
"Currently, we are validating these hits by testing their effect on the mouse lifespan. We hope that our biomarkers will significantly facilitate the search for new longevity interventions and help improve the healthspan and lifespan in rodents and, in the long term, in humans," says Alexander.
In addition to their scientific research, the scientists developed GENtervention, an application that offers fast and user-friendly tools for investigating the associations between the activity of individual genes and longevity.Source: https://www.domain-b.com/

Read More........→June 01, 2020 Categories: Scientist,University

Credit: James Strommer

There's a new disease-detecting technology in the lab of Sanjiv "Sam" Gambhir, MD PhD, and its No. 1 source of data is number one. And number two.

It's a smart toilet. But not the kind that lifts its own lid in preparation for use; this toilet is fitted with technology that can detect a range of disease markers in stool and urine, including those of some cancers, such as colorectal or urologic cancers. The device could be particularly appealing to individuals who are genetically predisposed to certain conditions, such as irritable bowel syndrome, prostate cancer or kidney failure, and want to keep on top of their health.

The smart toilet automatically sends data extracted from any sample to a secure, cloud-based system for safekeeping.

"Our concept dates back well over 15 years," said Gambhir, professor and chair of radiology. "When I'd bring it up, people would sort of laugh because it seemed like an interesting idea, but also a bit odd." With a pilot study of 21 participants now completed, Gambhir and his team have made their vision of a precision health-focused smart toilet a reality.

Gambhir's toilet is an ordinary toilet outfitted with gadgets inside the bowl. These tools, a suite of different technologies, use motion sensing to deploy a mixture of tests that assess the health of any deposits. Urine samples undergo physical and molecular analysis; stool assessment is based on physical characteristics.

The toilet automatically sends data extracted from any sample to a secure, cloud-based system for safekeeping. In the future, Gambhir said, the system could be integrated into any health care provider's record-keeping system for quick and easy access.

A paper describing the research will be published April 6 in Nature Biomedical Engineering. Gambhir is the senior author. Seung-min Park, PhD, senior research scientist; David Won, MD, PhD, former visiting scholar in the Molecular Imaging Program at Stanford; and postdoctoral scholar Brian Lee, PhD, share lead authorship.

Pulling double duty

The toilet falls into a category of technology known as continuous health monitoring, which encompasses wearables like smart watches. "The thing about a smart toilet, though, is that unlike wearables, you can't take it off," Gambhir said. "Everyone uses the bathroom -- there's really no avoiding it -- and that enhances its value as a disease-detecting device."

Although the idea may take some getting used to, Gambhir, who holds the Virginia and D.K. Ludwig Professorship for Clinical Investigation in Cancer Research, envisions the smart toilet as part of the average home bathroom. In facilitating that broad adaption, Gambhir designed the "smart" aspect as an add-on -- a piece of technology that's readily integrated into any old porcelain bowl. "It's sort of like buying a bidet add-on that can be mounted right into your existing toilet," he said. "And like a bidet, it has little extensions that carry out different purposes."

These extensions sport an array of health-monitoring technologies that look for signs of disease. Both urine and stool samples are captured on video and are then processed by a set of algorithms that can distinguish normal "urodynamics" (flow rate, stream time and total volume, among other parameters) and stool consistencies from those that are unhealthy.

Alongside physical stream analysis, the toilet also deploys uranalysis strips, or "dipstick tests," to measure certain molecular features. White blood cell count, consistent blood contamination, certain levels of proteins and more can point to a spectrum of diseases, from infection to bladder cancer to kidney failure. In its current stage of development, Gambhir said, the toilet can measure 10 different biomarkers.

It's still early days, though, with a total of 21 participants having tested the toilet over the course of several months. To get a better feel for "user acceptance" more broadly, the team surveyed 300 prospective smart-toilet users. About 37% said they were "somewhat comfortable" with the idea, and 15% said they were "very comfortable" with the idea of baring it all in the name of precision health.

ID please :

One of the most important aspects of the smart toilet may well be one of the most surprising -- and perhaps unnerving: It has a built-in identification system. "The whole point is to provide precise, individualized health feedback, so we needed to make sure the toilet could discern between users," Gambhir said. "To do so, we made a flush lever that reads fingerprints." The team realized, however, that fingerprints aren't quite foolproof. What if one person uses the toilet, but someone else flushes it? Or what if the toilet is of the auto-flush variety?

They added a small scanner that images a rather camera-shy part of the body. You might call it the polar opposite of facial recognition. In other words, to fully reap the benefits of the smart toilet, users must make their peace with a camera that scans their anus.

"We know it seems weird, but as it turns out, your anal print is unique," Gambhir said. The scans -- both finger and nonfinger -- are used purely as a recognition system to match users to their specific data. No one, not you or your doctor, will see the scans.

By no means is this toilet a replacement for a doctor, or even a diagnosis, Gambhir said. In fact, in many cases, the toilet won't ever report data to the individual user. In an ideal scenario, should something questionable arise -- like blood in the urine -- an app fitted with privacy protection would send an alert to the user's health care team, allowing professionals to determine the next steps for a proper diagnosis. The data would be stored in a secure, cloud-based system. Data protection, both in terms of identification and sample analyses, is a crucial piece of this research, Gambhir said. "We have taken rigorous steps to ensure that all the information is de-identified when it's sent to the cloud and that the information -- when sent to health care providers -- is protected under HIPAA," he said, referring to the Health Insurance Portability and Accountability Act, which restricts the disclosure of health care records.

Smart toilet 2.0

As Gambhir and his team continue to develop the smart toilet, they're focusing on a few things: increasing the number of participants, integrating molecular features into stool analysis and refining the technologies that are already working. They're even individualizing the tests deployed by the toilet. For example, someone with diabetes may need his or her urine monitored for glucose, whereas someone else who is predisposed to bladder or kidney cancer might want the toilet to monitor for blood.

Gambhir's other goal is to further develop molecular analysis for stool samples. "That's a bit trickier, but we're working toward it," Gambhir said. "The smart toilet is the perfect way to harness a source of data that's typically ignored -- and the user doesn't have to do anything differently."