The first pig kidney has been transplanted into a living person. But we’re still a long way from solving organ shortages

In a world first, we heard last week that US surgeons had transplanted a kidney from a gene-edited pig into a living human. News reports said the procedure was a breakthrough in xenotransplantation – when an organ, cells or tissues are transplanted from one species to another.

The world’s first transplant of a gene-edited pig kidney into a live human was announced last week.

Champions of xenotransplantation regard it as the solution to organ shortages across the world. In December 2023, 1,445 people in Australia were on the waiting list for donor kidneys. In the United States, more than 89,000 are waiting for kidneys.

One biotech CEO says gene-edited pigs promise “an unlimited supply of transplantable organs”.

Not, everyone, though, is convinced transplanting animal organs into humans is really the answer to organ shortages, or even if it’s right to use organs from other animals this way.

There are two critical barriers to the procedure’s success: organ rejection and the transmission of animal viruses to recipients.

But in the past decade, a new platform and technique known as CRISPR/Cas9 – often shortened to CRISPR – has promised to mitigate these issues.

What is CRISPR?

CRISPR gene editing takes advantage of a system already found in nature. CRISPR’s “genetic scissors” evolved in bacteria and other microbes to help them fend off viruses. Their cellular machinery allows them to integrate and ultimately destroy viral DNA by cutting it.

In 2012, two teams of scientists discovered how to harness this bacterial immune system. This is made up of repeating arrays of DNA and associated proteins, known as “Cas” (CRISPR-associated) proteins.

When they used a particular Cas protein (Cas9) with a “guide RNA” made up of a singular molecule, they found they could program the CRISPR/Cas9 complex to break and repair DNA at precise locations as they desired. The system could even “knock in” new genes at the repair site.

In 2020, the two scientists leading these teams were awarded a Nobel prize for their work.

In the case of the latest xenotransplantation, CRISPR technology was used to edit 69 genes in the donor pig to inactivate viral genes, “humanise” the pig with human genes, and knock out harmful pig genes.

How does CRISPR work?

A busy time for gene-edited xenotransplantation

While CRISPR editing has brought new hope to the possibility of xenotransplantation, even recent trials show great caution is still warranted.

In 2022 and 2023, two patients with terminal heart diseases, who were ineligible for traditional heart transplants, were granted regulatory permission to receive a gene-edited pig heart. These pig hearts had ten genome edits to make them more suitable for transplanting into humans. However, both patients died within several weeks of the procedures.

Earlier this month, we heard a team of surgeons in China transplanted a gene-edited pig liver into a clinically dead man (with family consent). The liver functioned well up until the ten-day limit of the trial.

How is this latest example different?

The gene-edited pig kidney was transplanted into a relatively young, living, legally competent and consenting adult.

The total number of gene edits edits made to the donor pig is very high. The researchers report making 69 edits to inactivate viral genes, “humanise” the pig with human genes, and to knockout harmful pig genes.

Clearly, the race to transform these organs into viable products for transplantation is ramping up.

From biotech dream to clinical reality

Only a few months ago, CRISPR gene editing made its debut in mainstream medicine.

In November, drug regulators in the United Kingdom and US approved the world’s first CRISPR-based genome-editing therapy for human use – a treatment for life-threatening forms of sickle-cell disease.

The treatment, known as Casgevy, uses CRISPR/Cas-9 to edit the patient’s own blood (bone-marrow) stem cells. By disrupting the unhealthy gene that gives red blood cells their “sickle” shape, the aim is to produce red blood cells with a healthy spherical shape.

Although the treatment uses the patient’s own cells, the same underlying principle applies to recent clinical xenotransplants: unsuitable cellular materials may be edited to make them therapeutically beneficial in the patient.

CRISPR technology is aiming to restore diseased red blood cells to their healthy round shape. Sebastian Kaulitzki/Shutterstock

We’ll be talking more about gene-editing

Medicine and gene technology regulators are increasingly asked to approve new experimental trials using gene editing and CRISPR.

However, neither xenotransplantation nor the therapeutic applications of this technology lead to changes to the genome that can be inherited.

For this to occur, CRISPR edits would need to be applied to the cells at the earliest stages of their life, such as to early-stage embryonic cells in vitro (in the lab).

In Australia, intentionally creating heritable alterations to the human genome is a criminal offence carrying 15 years’ imprisonment.

No jurisdiction in the world has laws that expressly permits heritable human genome editing. However, some countries lack specific regulations about the procedure.

Is this the future?

Even without creating inheritable gene changes, however, xenotransplantation using CRISPR is in its infancy.

For all the promise of the headlines, there is not yet one example of a stable xenotransplantation in a living human lasting beyond seven months.

While authorisation for this recent US transplant has been granted under the so-called “compassionate use” exemption, conventional clinical trials of pig-human xenotransplantation have yet to commence.

But the prospect of such trials would likely require significant improvements in current outcomes to gain regulatory approval in the US or elsewhere.

By the same token, regulatory approval of any “off-the-shelf” xenotransplantation organs, including gene-edited kidneys, would seem some way off.The Conversation

Christopher Rudge, Law lecturer, University of Sydney

This article is republished from The Conversation under a Creative Commons license. Read the original article.

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Scientists Have 3D-Scanned Thousands of Creatures Creating Incredibly Intricate Images Anyone Can Access for Free

3D scanned creatures by oVert – Released by Florida Museum of Natural History / SWNS
An incredible new project has scanned thousands of creatures to advance scientific research and provide colorful images to the world. Natural history museums have entered a new stage of scientific discovery and accessibility with the completion of openVertebrate (oVert), a five-year collaborative project among 18 institutions to create 3D reconstructions of vertebrate specimens and make them freely available online. Now, researchers have published a summary of the project in the journal BioScience reviewing the specimens they’ve scanned to date, offering a glimpse of how the data might be  used to ask newquestions and spur the development of innovative technology. “When people first collected these specimens, they had no idea what the future would hold for them,” said Edward Stanley, co-principal investigator of the oVert project and associate scientist at the Florida Museum of Natural History. Such museums got their start in the 16th century as cabinets of curiosity, in which a few wealthy individuals amassed rare and exotic specimens, which they kept mostly to themselves. Since then, museums have become a resource for the public to learn about biodiversity. But, the majority of museum collections remain behind closed doors—accessible only to scientists who must either travel to see them or ask that a small number of specimens be mailed on loan—and oVert wants to change that. “Now we have scientists, teachers, students and artists around the world using these data remotely,” said David Blackburn, lead principal investigator of the oVert project and curator of herpetology at the Florida Museum. Beginning in 2017, the oVert team members took CT scans of more than 13,000 specimens, with vertebrate species across the tree of life, including over half the genera of all amphibians, reptiles, fishes, and mammals.
A collage of scanned fish from oVert – Released by Florida Museum of Natural History / SWNS
CT scanners use high-energy X-rays to peer past an organism’s exterior and view the dense bone structure beneath. Some specimens were also stained with a contrast-enhancing solution for visualizing soft tissues, like skin, muscle, and other organs. The models give an intimate look at internal portions of a specimen that could previously only be observed through destructive dissection and tissue sampling. “You want to protect specimens, but you also want to have people use them,” Blackburn said. “oVert is a way of reducing the wear and tear on samples while also increasing access, and it’s the next logical step in the
Hedgehog CT scan from oVert – Florida Museum of Natural History / SWNS
mission of museum collections.” Skeletons too large to fit into a CT scanner, like a humpback whale, were painstakingly taken apart so that 3D models of each individual bone could be scanned and reassembled. “These are not things you put in boxes and loan,” Blackburn pointed out. A set of iconic Galapagos tortoises at the California Academy of Sciences were each photographed in a 360-degree rotation. Photographing their undersides was problematic, as their curved shells made it impossible to keep them upright. After a few trial-and-error runs, they settled on placing the specimens on top of inflatable swimming tubes. Scientists have already used data from the project to gain astonishing insights into the natural world. Watch the incredible video below, and learn more at the bottom…In 2023, Edward Stanley was conducting routine CT scans of spiny mice and was surprised to find their tails were covered with an internal coat of bony plates, called osteoderms. Before this discovery, armadillos were considered to be the only living mammals with these structures. “All kinds of things jump out at you when you’re when you’re scanning,” Stanley said. “I study osteoderms, and through kismet or fate, I happened to be the one scanning those particular specimens on that particular day and noticed something strange about their tails on the X-ray. “That happens all the time. We’ve found all sorts of strange, unexpected things.”oVert scans were used to determine what killed a rim rock crown snake, considered to be the rarest snake species in North America. Another study showed that a group of frogs called pumpkin toadlets had become so small that the fluid-filled canals in their ears that confer balance no longer functioned properly, causing them to crash-land when jumping. One study of 500 oVert specimens revealed that frogs have lost and regained teeth more than 20 times throughout their evolutionary history. Other researchers concluded that Spinosaurus, a massive dinosaur that was larger than Tyrannosaurus rex and thought to be aquatic, would have actually been a poor swimmer, and thus likely stayed on land. And the list goes on, full of insights and ideas that would have been impossible or impractical before the project’s outset. “Now that we’ve been working on this for so long, we have a broad scaffold that allows us to take a broader view of
Fish CT scan from oVert – Florida Museum of Natural History / SWNS
evolutionary questions,” Stanley said. Artists and teachers are benefitting too Funded in part by the National Science Foundation, the value of the oVert project extends beyond science. Artists have used the 3D models to create realistic animal replicas, photographs of oVert specimens have been displayed as museum exhibits, and specimens have been incorporated into virtual reality headsets that give users the chance to interact with and manipulate them. A high school teacher in Cincinnati says it’s been a game-changer for her studies on evolution. “I teach juniors and seniors, and I absolutely love them, but they can be a tough audience,” said Jennifer Broo. “They know when things are fake, which makes them less engaged. Using the oVert models, my class has gotten so much better because I have had the opportunities to work with and expose my students to real data.”Visit Sketchfab to view a sample of 3D interactive models. At MorphoSource you can access the full openVertebrate repository.Scientists Have 3D-Scanned Thousands of Creatures Creating Incredibly Intricate Images Anyone Can Access for Free
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The US just returned to the Moon after more than 50 years. How big a deal is it, really?

In the few short years since the COVID pandemic changed our world, China, Japan and India have all successfully landed on the Moon.

Many more robotic missions have flown past the Moon, entered lunar orbit, or crashed into it in the past five years. This includes spacecraft developed by South Korea, the United Arab Emirates, and an Israeli not-for-profit organisation.

Late last week, the American company Intuitive Machines, in collaboration with NASA, celebrated “America’s return to the Moon” with a successful landing of its Odysseus spacecraft.

Recent Chinese-built sample return missions are far more complex than this project. And didn’t NASA ferry a dozen humans to the Moon back when microwaves were cutting-edge technology? So what is different about this mission developed by a US company?

Back to the Moon

The recent Odysseus landing stands out for two reasons. For starters, this is the first time a US-built spacecraft has landed – not crashed – on the Moon for over 50 years.

Secondly, and far more significantly, this is the first time a private company has pulled off a successful delivery of cargo to the Moon’s surface.

NASA has lately focused on destinations beyond the Earth–Moon system, including Mars. But with its Commercial Lunar Payload Services (CLPS) program, it has also funded US private industry to develop Moon landing concepts, hoping to reduce the delivery costs of lunar payloads and allow NASA engineers to focus on other challenges.

Working with NASA, Intuitive Machines selected a landing site about 300 kilometres from the lunar south pole. Among other challenges, landing here requires entering a polar orbit around the Moon, which consumes additional fuel.

At this latitude, the land is heavily cratered and dotted with long shadows. This makes it challenging for autonomous landing systems to find a safe spot for a touchdown.

NASA spent about US$118 million (A$180 million) to land six scientific payloads on Odysseus. This is relatively cheap. Using low-cost lunar landers, NASA will have an efficient way to test new space hardware that may then be flown on other Moon missions or farther afield.

Ten minutes of silence

One of the technology tests on the Odysseus lander, NASA’s Navigation Doppler Lidar experiment or NDL, appears to have proved crucial to the lander’s success.

As the lander neared the surface, the company realised its navigation systems had a problem. NASA’s NDL experiment is serendipitously designed to test precision landing techniques for future missions. It seems that at the last second, engineers bodged together a solution that involved feeding necessary data from NDL to the lander.

Ten minutes of silence followed before a weak signal was detected from Odysseus. Applause thundered through the mission control room. NASA’s administrator released a video congratulating everyone for returning America to the Moon.

It has since become clear the lander is not oriented perfectly upright. The solar panels are generating sufficient power and the team is slowly receiving the first images from the surface.

However, it’s likely Odysseus partially toppled over upon landing. Fortunately, at the time of writing, it seems most of the science payload may yet be deployed as it’s on the side of the lander facing upwards. The unlucky payload element facing downwards is a privately contributed artwork connected to NFTs.

The lander is now likely to survive for at least a week before the Sun sets on the landing site and a dark, frigid lunar night turns it into another museum piece of human technology frozen in the lunar regolith.

The Moon visible 10km beneath the Odysseus lander after it entered lunar orbit on February 21. Intuitive Machines, CC BY-NC-ND

Win some, lose some

NASA’s commercial approach to stimulating low-cost payload services all but guarantees some failures. But eventually NASA hopes that several commercial launch and landing providers will emerge from the program, along with a few learning experiences.

The know-how accumulated at organisations operating hardware in space is at least as important as the development of the hardware itself.

The market for commercial lunar payloads remains unclear. Possibly, once the novelty wears off and brands are no longer able to generate buzz by, for example, sending a piece of outdoor clothing to the Moon, this source of funding may dwindle.

However, just as today, civil space agencies and taxpayers will continue to fund space exploration to address shared science goals.

Ideally, commercial providers will offer NASA an efficient method for testing key technologies needed for its schedule of upcoming scientific robotic missions, as well as human spaceflight in the Artemis program. Australia would also have the opportunity to test hardware at a reduced price.

It’s worth noting that US budgetary issues, funding cuts and subsequent lay-offs do threaten these ambitions.

Meanwhile, in Australia, we may have nothing to launch anyway. We continue to spend less than the OECD average on scientific research, and only a few Australian universities – who traditionally lead such efforts – have received funding provided by the Australian Space Agency.

If we do support planetary science and space exploration in the future, Australians will need to decide if we want to allocate our limited resources, competing with NASA and US private industry, to supply launch, landing and robotic services to the global space industry.

Alternatively, we could leverage these lower-cost payload providers to develop our own scientific space program, and locally developed space technologies associated with benefits to the knowledge economy, education and national security.The Conversation

David Flannery, Planetary Scientist, Queensland University of Technology

This article is republished from The Conversation under a Creative Commons license. Read the original article.

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Atlanta Science Festival returns to inspire discovery for all ages

A middle-school student experiences an Emory chemistry lab during a recent community outreach event.
The Atlanta Science Festival returns March 9 to 23, inviting curious kids and adults to explore all things science, technology, engineering and mathematics (STEM). Experts in these fields — including many members of the Emory community — will serve as educational guides for more than 150 interactive events. “The Atlanta Science Festival aims to bring the community together through their shared love of science,” says Meisa Salaita, co-founder and co-executive director of Science ATL, the engineers of the festival. “Through these events, we hope to inspire and empower the next generation to pursue their dreams.” Participants can take a crash course on the basics of AI, create an herbarium of medicinal plants, go into the field with researchers studying microplastic pollution in a stream, take a behind-the-scenes tour of the latest advances in healthcare technology and even get a taste of the physics of cheese making. Now in its 11th year, the Atlanta Science Festival was co-founded by Emory, Georgia Tech and the Metro Atlanta Chamber. “We have grown into a mainstay of Atlanta,” says Salaita, noting that many of the events fill up quickly. “The festival is something that people look forward to every spring.” Click here for highlights of this year's festival with an Emory connection. eScienceCommons: Atlanta Science Festival returns to inspire discov.
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Cultivated Biosciences secures US$5 million seed funding amid US market launch in 2025

(Image credit: Cultivated Biosciences)
07 Mar 2024 --- Cultivated Biosciences, a Swiss biotech start-up, has recently announced the closure of its US$5 million seed funding round. This financial milestone will boost the development of its innovative yeast cream in collaboration with the food industry, setting the stage for a US market launch slated for 2025. The latest funding round attracted a group of investors led by Navus Ventures, a Dutch venture capital firm with a focus on sustainable food and energy systems. The investors were drawn to the company’s progress following a US$1.5 million pre-seed round in September 2022. “This funding validates our innovative approach and enables us to introduce our revolutionary yeast cream to the market,” says Tomas Turner, Cultivated Biosciences’ CEO. “We aim to make alternative dairy products appealing to traditional dairy consumers, not just to reduce CO2 emissions from dairy production but also to tap into a multi-billion dollar market opportunity.” Innovative yeast cream: In the dairy-free sector, efforts to replicate the dairy experience involve utilizing plant-based proteins combined with vegetable oils and various additives such as emulsifiers and texturizers. These attempts aim to recreate both the sensory and functional characteristics of dairy products. Cultivated Biosciences is innovating on a different approach. Its yeast cream is a natural emulsion produced through yeast biomass fermentation. This method is claimed to be non-GMO, more cost-effective, and easier to scale than precision fermentation. According to the company, this ingredient is patented and improves the stability of dairy-free products, replaces additives, and doesn’t affect taste. The yeast cream has fats, proteins, and fibers, all derived from yeast, and features a microstructure that mirrors milk fat droplets, allowing it to blend into various consumer products such as coffee creamers, milk, and ice cream. Enter the market: Cultivated Biosciences was a participant in the ProVeg Incubator program, which focused specifically on emerging food technologies and novel ingredients for the alternative protein sector. In the latest interview with Food Ingredients First, Antje Räuscher, programme & innovation manager, and Albrecht Wolfmeyer, international director from ProVeg, shared about the rising global demand for plant-based diets. This growing market aligns with the increasing consumer demand for health- and environmentally-conscious products. Cultivated Biosciences plans to launch in the US market next year and the European market in 2026, pending regulatory approvals.Edited by Sichong Wang. Cultivated Biosciences secures US$5 million seed funding amid US market launch in 2025
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