Scientists Discover Potential HIV Cure that Eliminates Disease from Cells Using CRISPR-Cas Gene Editing

HIV-1 virus particles under electron micrograph with H9 T-cells (in blue) – Credit: National Institute of Allergy and Infectious Diseases
A new study has unveiled a likely future cure for HIV which uses molecular scissors to ‘cut out’ HIV DNA from infected cells. To cut out this virus, the team used CRISPR-Cas gene editing technology—a groundbreaking method that allows for precise alterations to a patient’s genome, for which its inventors won the Nobel Prize in Chemistry in 2020. One of the significant challenges in HIV treatment is the virus’s ability to integrate its genome into the host’s DNA, making it extremely difficult to eliminate—but the CRISPR-Cas tool provides a new means to isolate and target HIV DNA. Because HIV can infect different types of cells and tissues in the body, each with its own unique environment and characteristics, the researchers are searching for a way to target HIV in all of these situations. In this study, which is to be presented ahead of this year’s European Congress of Clinical Microbiology and Infectious Diseases, the authors used CRISPR-Cas and two guide RNAs against “conserved” HIV sequences. They focused on parts of the virus genome that stay the same across all known HIV strains and infected T cells. Their experiments showed outstanding antiviral performance, managing to completely inactivate HIV with a single guide RNA and cut out the viral DNA with two guide RNAs. “We have developed an efficient combinatorial CRISPR-attack on the HIV virus in various cells and the locations where it can be hidden in reservoirs, and demonstrated that therapeutics can be specifically delivered to the cells of interest,” said Associate professor Elena Herrera Carrillo from the University of Amsterdam AMC. “These findings
HIV AIDS virus (in yellow) infecting a human cell – Credit: National Cancer Institute
represent a pivotal advancement towards designing a cure strategy.” The team has a long way to go before their cure will be available to patients, but said, “These preliminary findings are very encouraging’. Currently, HIV can be kept in check with anti-retroviral medication, but no one has actually been cured—although three patients receiving stem cell transplants for blood cancer were subsequently declared free of the disease when their HIV became undetectable. “We hope to achieve the right balance between efficacy and safety of this CURE strategy,” said Dr. Carrillo. “Only then can we consider clinical trials of ‘cure’ in humans to disable the HIV reservoir.“Our aim is to develop a robust and safe combinatorial CRISPR-Cas regimen, striving for an inclusive ‘HIV cure for all’ that can inactivate diverse HIV strains across various cellular contexts. Scientists Discover Potential HIV Cure that Eliminates Disease from Cells Using CRISPR-Cas Gene Editing
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Bobi, known as the oldest dog in the world, dies aged 31

Bobi, who was this year ordained the oldest dog in the world by Guinness World Records, has died at the ripe old age of 31, Portuguese media reported Monday.

"We have better memories of a long life where he was happy and, above all, where he made a lot of people happy, especially his family," Bobi's owner Leonel Costa told local media from the little village in central Portugal where he lives.

A purebreed Rafeiro, a Portuguese livestock guard dog whose normal life expectancy is between 12 and 14, Bobi was not supposed to make it beyond puppyhood.

He was born on May 11, 1992, along with three other pups in a wood storage shed owned by the Costa family in the village of Conqueiros.

Because the family owned so many animals, the father decided they could not keep the newborn puppies and the parents took them from the shed the next day, while the mother dog Gira was out, said Leonel Costa, who was eight years old at the time.

But they didn't realise they had left one puppy behind, and that puppy became Bobi.

"He died at the age of 31 years and 165 days," according to the Guinness World Records.After he was declared the world's oldest dog in February, media and curious onlookers from around the world paid Bobi a visit. Bobi, known as the oldest dog in the world, dies aged 31
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Walking robot tested in Finnish repository : Corporate

The ANYmal robot walks through Onkalo's underground tunnels (Image: Tapani Karjanlahti / Posiva)
A four-legged robot designed for autonomous operation in challenging environments has been put through its paces at a depth of more than 400 metres in the tunnels of the Onkalo underground used nuclear fuel repository near Olkiluoto, Finland.

A research team led by the Swiss robotics company ANYbotics visited Olkiluoto in June to test the functionality of its ANYmal robot in underground facilities. The test was organised by Euratom - the European Atomic Energy Community - together with Finnish radioactive waste management company Posiva Oy.

‍The ANYmal robot has been under development for many years. The roots of the ANYbotics company go back to the Swiss Institute of Technology, EHT. A group of researchers from the educational institution built the first four-legged robot back in 2009, and ANYbotics was founded for the commercialisation of this technology in 2016.

The ANYmal robot uses laser sensors and cameras to observe the environment and can locate its own position very precisely. By combining observation data with location data - such as a map or area scan data - it can plan its navigation route independently when necessary.

Posiva said Onkalo offered a unique framework for the robot to move, noting that there are tunnels in other parts of the world, but no other underground disposal facility has yet been built.

During the test, the robot - measuring 93cm in length, 53cm in width and 89cm in height and weighing about 50kg - travelled through the tunnels of Onkalo for about 1.5 hours. With a fully-charged battery, the robot can operate for up to 2 hours. The purpose was to test how far the robot can travel in Onkalo conditions with one charge, and whether there are any terrains in the tunnel where the robot would not be able to advance.

For the test, the robot first "walked" the planned route by remote control, and scanned the map into its internal system. In the test itself, the robot moved along the scanned route autonomously, although all the time in the line of sight with the research team. It was also available for remote control at any moment, for example in case of danger. Various safety functions were programmed into the robot. For example, it went around the obstacles on the route from a certain safety distance and stopped when something came into its safety area.

Authorities are interested in the use of robots for the reason that a robot can reach places that are inaccessible to humans, for example for nuclear material protection inspection work. Carrying out nuclear safeguards with the help of a robot is also of interest to Posiva, the company said. Robots can also be used in rescue operations and industry. They can be equipped with different devices for different tasks, such as optical and thermal cameras, microphones, gas or radiation detectors.

A video of the ANYmal robot in Onkalo can be found here.Researched and written by World Nuclear News  Source: - World Nuclear News
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How consciousness may rely on brain cells acting collectively – new psychedelics research on rats

Psychedelics can help uncover consciousness. agsandrew/Shutterstock Pär Halje, Lund University
Psychedelics are known for inducing altered states of consciousness in humans by fundamentally changing our normal pattern of sensory perception, thought and emotion. Research into the therapeutic potential of psychedelics has increased significantly in the last decade. While this research is important, I have always been more intrigued by the idea that psychedelics can be used as a tool to study the neural basis of human consciousness in laboratory animals. We ultimately share the same basic neural hardware with other mammals, and possibly some basic aspects of consciousness, too. So by examining what happens in the brain when there’s a psychedelically induced change in conscious experience, we can perhaps glean insights into what consciousness is in the first place.We still don’t know a lot about how the networks of cells in the brain enable conscious experience. The dominating view is that consciousness somehow emerges as a collective phenomenon when the dispersed information processing of individual neurons (brain cells) is integrated as the cells interact.But the mechanism by which this is supposed to happen remains unclear. Now our study on rats, published in Communications Biology, suggests that psychedelics radically change the way that neurons interact and behave collectively.Our study compared two different classes of psychedelics in rats: the classic LSD type and the less-typical ketamine type (ketamine is an anaesthetic in larger doses). Both classes are known to induce psychedelic experiences in humans, despite acting on different receptors in the brain. Exploring brain waves: We used electrodes to simultaneously measure electrical activity from 128 separate areas of the brain of nine awake rats while they were given psychedelics. The electrodes could pick up two kinds of signals: electrical brain waves caused by the cumulative activity in thousands of neurons, and smaller transient electrical pulses, called action potentials, from individual neurons. The classic psychedelics, such as LSD and psilocybin (the active ingredient in magic mushrooms), activates a receptor in the brain (5-HT2A) which normally binds to serotonin, a neurotransmitter that regulates mood and many other things. Ketamine, on the other hand, works by inhibiting another receptor (NMDA), which normally is activated by glutamate, the primary neurotransmitter in the brain for making neurons fire. We speculated that, despite these differences, the two classes of psychedelics might have similar effects on the activity of brain cells. Indeed, it turned out that both drug classes induced a very similar and distinctive pattern of brain waves in multiple brain regions. The brain waves were unusually fast, oscillating about 150 times per second. They were also surprisingly synchronised between different brain regions. Short bursts of oscillations at a similar frequency are known to occur occasionally under normal conditions in some brain
Brain waves on electroencephalogram EEG. Chaikom/Shutterstock
regions. But in this case, it occurred for prolonged durations.  First, we assumed that a single brain structure was generating the wave and that it then spread to other locations. But the data was not consistent with that scenario. Instead, we saw that the waves went up and down almost simultaneously in all parts of the brain where we could detect them – a phenomenon called phase synchronisation. Such tight phase synchronisation over such long distances has to our knowledge never been observed before. We were also able to measure action potentials from individual neurons during the psychedelic state. Action potentials are electrical pulses, no longer than a thousandth of a second, that are generated by the opening and closing of ion channels in the cell membrane. The action potentials are the primary way that neurons influence each other. Consequently, they are considered to be the main carrier of information in the brain. However, the action potential activity caused by LSD and ketamine differed significantly. As such, they could not be directly linked to the general psychedelic state. For LSD, neurons were inhibited – meaning they fired fewer action potentials – in all parts of the brain. For ketamine, the effect depended on cell type – certain large neurons were inhibited, while a type of smaller, locally connecting neurons, fired more. Therefore, it is probably the synchronised wave phenomenon – how the neurons behave collectively – that is most strongly linked to the psychedelic state. Mechanistically, this makes some sense. It is likely that this type of increased synchrony has large effects on the integration of information across neural systems that normal perception and cognition rely on. I think that this possible link between neuron-level system dynamics and consciousness is fascinating. It suggests that consciousness relies on a coupled collective state rather than the activity of individual neurons – it is greater than the sum of its parts. That said, this link is still highly speculative at this point. That’s because the phenomenon has not yet been observed in human brains. Also, one should be cautious when extrapolating human experiences to other animals – it is of course impossible to know exactly what aspects of a trip we share with our rodent relatives. But when it comes to cracking the deep mystery of consciousness, every bit of information is valuable. Pär Halje, Associate Research Fellow of Neurophysiology, Lund University This article is republished from The Conversation under a Creative Commons license. Read the original article.
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European Space Agency's Euclid telescope launches from Florida, US

An artist's impression of Euclid. Image: ESA.
Yesterday, a SpaceX Falcon 9 rocket, with the European Space Agency's (ESA) Euclid space telescope aboard, launched at 11:12 AM EDT (1512 UTC) from Cape Canaveral Space Launch Complex 40 in Florida, US. Euclid was to study dark matter, dark energy, and the expansion of the universe. Costing 1.4 billion, Euclid was to spend about a month traveling around 1,500,000 kilometers (932,057 mi) to the Lagrange point L2 between the Earth and the Sun, the area of the James Webb Space Telescope. There, it would observe about a third of the sky beyond the Milky Way for six years. NASA designed and built Euclid's Near Infrared Spectrometer and Photometer, and NASA's Nancy Grace Roman Space Telescope, with a tentative launch date of May 2027, was to provide more refined data scientists could use to correct Euclid's. IPAC senior research scientist Yun Wang stated Euclid and Roman would "add up to much more than the sum of their parts [...] Combining their observations will give astronomers a better sense of what's actually going on in the universe." Before the Russian invasion of Ukraine, Euclid was scheduled for launch from French Guiana on a Russian Soyuz rocket in March 2023. Source: https://en.wikinews.org, available under the terms of the Creative Commons Attribution 2.5 License
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